making a Duilution series

Aim: To make a dilution series to investigate concentration

Equipment: A potassium permanganate crystal, six large test tubes, tweezers, a plastic transfer pipette, a test tube rack, 10,l measuring cylinder.

Method:

1. Place the six test tubes in a test tube rack. Label the rack with numbers 1-6.

2. Using the measuring cylinder, fill test tube 1 with 10 ML of water. Fill the remaining test tubes with 5 ML of water.

3. Using your tweezers, add a single crystal of potassium permanganate to test tube 1.

4. gently shake the test tube until the Crystal has dissolved

5.Using the transfer pipette. carefully remove exact,y 4 ML from test tube 1 and pour it into test tube 2

6. Rinse the transfer pipette thoroughly to ensure that no purple solution remains.

7. gently shake test tube 2 and repeat the transfer process, transferring exactly 5 ml of solution from test tube 2 to test tube 3

8. Rinse the pipette again and repeat the transfer process for test tubes 4, 5 and 6